› 7bgu

X-ray diffraction
2.43Å resolution

Mason-Pfizer Monkey Virus Protease mutant C7A/D26N/C106A in complex with peptidomimetic inhibitor

Released:
DOI: 10.2210/pdb7bgu/pdb
PDB entry 7bgu coloured by chain, front view.
PDB entry 7bgu coloured by chain, side view.
PDB entry 7bgu coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of inhibitor complexes of M-PMV protease with visible flap loops.
Wosicki S, Kazmierczyk M, Gilski M, Zabranska H, Pichova I, Jaskolski M
OpenAccess logo Protein Sci 30 1258-1263 (2021)
PMID: 33786913

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139640 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protease 13 kDa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 12.9 KDa
Source organism: Mason-Pfizer monkey virus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P07572 (Residues: 760-873; Coverage: 6%)
Gene name: gag-pro-pol
Sequence domains: Retroviral aspartyl protease
peptidomimetic inhibitor Chains: F, G
Molecule details ›
Chains: F, G
Length: 7 amino acids
Theoretical weight: 886 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand 1PE 2 x 1PE
1 modified residue:
Ligand 0A1 2 x 0A1

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P1
Unit cell:
a: 29.068Å b: 67.893Å c: 69.739Å
α: 77.07° β: 83.34° γ: 83.18°
R-values:
R R work R free
0.182 0.18 0.236
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

1 review citation

Precursors of Viral Proteases as Distinct Drug Targets.
Majerová et al. (2021)

1 mention without citation

Crystal structures of inhibitor complexes of M-PMV protease with visible flap loops.
Wosicki et al. (2021)