› 7bph

X-ray diffraction
1.57Å resolution

Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13

Released:
DOI: 10.2210/pdb7bph/pdb
PDB entry 7bph coloured by chain, front view.
PDB entry 7bph coloured by chain, side view.
PDB entry 7bph coloured by chain, top view.
Source organisms:
Primary publication:
State-selective modulation of heterotrimeric Gαs signaling with macrocyclic peptides.
Dai SA, Hu Q, Gao R, Blythe EE, Touhara KK, Peacock H, Zhang Z, von Zastrow M, Suga H, Shokat KM
OpenAccess logo Cell (2022)
PMID: 36170854

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 44.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 7-394; Coverage: 95%)
  • Best match: P63092-2 (Residues: 7-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
GN13 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.63 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand GOL 1 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand DTY 1 x DTY

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 68.905Å b: 78.332Å c: 80.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.229
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Recommended Tool Compounds: Application of YM-254890 and FR900359 to Interrogate Gαq/11-Mediated Signaling Pathways.
Voss JH. (2023)
1 more

5 mentions without citation

Classification and energetics of the base-phosphate interactions in RNA.
Zirbel et al. (2009)
4 more