› 7car

X-ray diffraction
1.98Å resolution

Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: free+IPP)

Released:
DOI: 10.2210/pdb7car/pdb
PDB entry 7car coloured by chain, front view.
PDB entry 7car coloured by chain, side view.
PDB entry 7car coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Primary publication:
A versatile cis-prenyltransferase from Methanosarcina mazei catalyzes both C- and O-prenylations.
Okada M, Unno H, Emi KI, Matsumoto M, Hemmi H
OpenAccess logo J Biol Chem 296 100679
PMID: 33872599

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.69 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0F8R9A0 (Residues: 3-224; Coverage: 99%)
Gene names: DU31_20070, DU34_02440, DU35_05415, DU36_19645, DU37_02620, DU38_19395, DU39_01195, DU40_09340, DU41_00685, DU42_06650, DU44_12390, DU46_07955, DU48_11585, DU49_19880, DU50_02180, DU51_08075, DU54_06805, DU55_10290, DU57_09005, DU59_09550, DU60_00605, DU61_05980, DU62_08385, DU63_18660, DU65_11890, DU69_08085, DU71_03655, DU72_03110, DU73_16595, DU74_06630, DU75_19005, DU76_06945, DU77_03205, DU78_04790, DU79_15230, DU81_02685, DU82_17965, DU83_00555, DU84_03240, DU85_20370, DU86_04305, DU88_02360, FQU78_07000
Sequence domains: Putative undecaprenyl diphosphate synthase

Ligands and Environments

3 bound ligands:
Ligand IPE 1 x IPE
Ligand PO4 2 x PO4
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 79.94Å b: 100.42Å c: 129.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.259
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Complexation and evolution of cis-prenyltransferase homologues in Cinnamomum kanehirae deduced from kinetic and functional characterizations.
Liu et al. (2023)

1 mention without citation

A versatile cis-prenyltransferase from Methanosarcina mazei catalyzes both C- and O-prenylations.
Okada et al. (2021)