› 7d1x

X-ray diffraction
2.5Å resolution

Crystal structure of 7-alpha-hydroxyl bile acid sulfotransferase (Sult2a8)

Released:
DOI: 10.2210/pdb7d1x/pdb
PDB entry 7d1x coloured by chain, front view.
PDB entry 7d1x coloured by chain, side view.
PDB entry 7d1x coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Au WNS, Wang K

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + glycochenodeoxycholate = adenosine 3',5'-bisphosphate + glycochenodeoxycholate 7-sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-567920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 2A8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 33.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli str. 'clone D i14'
UniProt:
  • Canonical: Q8BGL3 (Residues: 4-282; Coverage: 99%)
Gene names: 2810007J24Rik, Sult2a8
Sequence domains: Sulfotransferase domain

Ligands and Environments

2 bound ligands:
Ligand CHD 2 x CHD
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P42
Unit cell:
a: 93.87Å b: 93.87Å c: 71.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.238 0.272
Expression system: Escherichia coli str. 'clone D i14'

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