› 7dmr

X-ray diffraction
2.2Å resolution

Crystal structure of potassium induced heme modification in yak lactoperoxidase at 2.20 A resolution

Released:
DOI: 10.2210/pdb7dmr/pdb
PDB entry 7dmr coloured by chain, front view.
PDB entry 7dmr coloured by chain, side view.
PDB entry 7dmr coloured by chain, top view.
Source organism: Bos mutus
Primary publication:
Potassium-induced partial inhibition of lactoperoxidase: structure of the complex of lactoperoxidase with potassium ion at 2.20 Å resolution.
Singh PK, Pandey S, Rani C, Ahmad N, Viswanathan V, Sharma P, Kaur P, Sharma S, Singh TP
J Biol Inorg Chem 26 149-159 (2021)
PMID: 33427997

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lactoperoxidase Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 67.77 KDa
Source organism: Bos mutus
UniProt:
  • Canonical: L8ICE9 (Residues: 118-712; Coverage: 86%)
Gene name: M91_18575
Sequence domains: Animal haem peroxidase

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand NAG 3 x NAG
Ligand CA 1 x CA
Ligand ZN 2 x ZN
Ligand CL 1 x CL
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: P212121
Unit cell:
a: 80.24Å b: 85.31Å c: 98.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.243

Quick links

Citations

1 citations in other articles

Structural evidence of the oxidation of iodide ion into hyper-reactive hypoiodite ion by mammalian heme lactoperoxidase.
Singh et al. (2022)