› 7ex7

X-ray diffraction
2.3Å resolution

Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 16

Released:
DOI: 10.2210/pdb7ex7/pdb
PDB entry 7ex7 coloured by chain, front view.
PDB entry 7ex7 coloured by chain, side view.
PDB entry 7ex7 coloured by chain, top view.
Source organism: Ebinur lake virus
Primary publication:
Structural and Biochemical Basis for Development of Diketo Acid Inhibitors Targeting the Cap-Snatching Endonuclease of the Ebinur Lake Virus (Order: Bunyavirales).
Kuang W, Zhang H, Cai Y, Zhang G, Deng F, Li H, Zhou Y, Wang M, Gong P, Guo Y, Hu Z
J Virol 96 e0217321 (2022)
PMID: 35266805

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 219 amino acids
Theoretical weight: 25.18 KDa
Source organism: Ebinur lake virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A059WLS9 (Residues: 1-183; Coverage: 8%)
Gene name: RdRp
Sequence domains: L protein N-terminus

Ligands and Environments

3 bound ligands:
Ligand MN 8 x MN
Ligand 1I6 4 x 1I6
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 70.272Å b: 41.676Å c: 145.305Å
α: 90° β: 102.03° γ: 90°
R-values:
R R work R free
0.211 0.208 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Antiviral Activity of Acetylsalicylic Acid against Bunyamwera Virus in Cell Culture.
Fernández-Sánchez et al. (2023)

1 mention without citation

Structural and Biochemical Basis for Development of Diketo Acid Inhibitors Targeting the Cap-Snatching Endonuclease of the Ebinur Lake Virus (Order: Bunyavirales).
Kuang et al. (2022)