› 7exh

X-ray diffraction
2.63Å resolution

Crystal structure of D383A mutant from Arabidopsis thaliana complexed with Galactinol.

Released:
DOI: 10.2210/pdb7exh/pdb
PDB entry 7exh coloured by chain, front view.
PDB entry 7exh coloured by chain, side view.
PDB entry 7exh coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insight into the hydrolase and synthase activities of an alkaline α-galactosidase from Arabidopsis from complexes with substrate/product.
Chuankhayan P, Lee RH, Guan HH, Lin CC, Chen NC, Huang YC, Yoshimura M, Nakagawa A, Chen CJ
OpenAccess logo Acta Crystallogr D Struct Biol 79 154-167 (2023)
PMID: 36762861

Function and Biology Details

Reaction catalysed: 
Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable galactinol--sucrose galactosyltransferase 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 749 amino acids
Theoretical weight: 83.2 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RX87 (Residues: 1-749; Coverage: 100%)
Gene names: At5g20250, DIN10, F5O24.140, RFS6, RS6
Sequence domains: Raffinose synthase or seed imbibition protein Sip1

Ligands and Environments

1 bound ligand:
Ligand BQZ 2 x BQZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 97.52Å b: 103.649Å c: 182.432Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.246
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Insights into Sucrose Metabolism and Its Ethylene-Dependent Regulation in Cucumis melo L.
Lao et al. (2023)

1 mention without citation

Structural insight into the hydrolase and synthase activities of an alkaline α-galactosidase from Arabidopsis from complexes with substrate/product.
Chuankhayan et al. (2023)