› 7f0y

X-ray diffraction
1.6Å resolution

Crystal structure of isomerase NsrQ F58A in complex with substrate analogue

Released:
DOI: 10.2210/pdb7f0y/pdb
PDB entry 7f0y coloured by chain, front view.
PDB entry 7f0y coloured by chain, side view.
PDB entry 7f0y coloured by chain, top view.
Source organism: Aspergillus novofumigatus
Primary publication:
Structural Basis for Isomerization Reactions in Fungal Tetrahydroxanthone Biosynthesis and Diversification.
Yang J, Mori T, Wei X, Matsuda Y, Abe I
Angew Chem Int Ed Engl 60 19458-19465 (2021)
PMID: 34180120

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monooxygenase nsrQ Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 19.15 KDa
Source organism: Aspergillus novofumigatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2I1C3W8 (Residues: 1-151; Coverage: 100%)
Gene names: P174DRAFT_407796, nsrQ

Ligands and Environments

1 bound ligand:
Ligand 0BJ 2 x 0BJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 57.574Å b: 64.633Å c: 87.037Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.214
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Branching and converging pathways in fungal natural product biosynthesis.
Wei et al. (2022)
1 more