› 7fdz

X-ray diffraction
1.35Å resolution

Levansucrase from Brenneria sp. EniD 312 with sucrose

Released:
DOI: 10.2210/pdb7fdz/pdb
PDB entry 7fdz coloured by chain, front view.
PDB entry 7fdz coloured by chain, side view.
PDB entry 7fdz coloured by chain, top view.
Source organism: Brenneria sp. EniD312
Primary publication:
Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity.
Xu W, Ni D, Hou X, Pijning T, Guskov A, Rao Y, Mu W
J Agric Food Chem 70 5095-5105 (2022)
PMID: 35388691

Function and Biology Details

Reaction catalysed: 
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
levansucrase Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 49.4 KDa
Source organism: Brenneria sp. EniD312
Expression system: Escherichia coli
UniProt:
  • Canonical: G7LSK3 (Residues: 1-437; Coverage: 100%)
Gene name: BrE312_3941
Sequence domains: Levansucrase/Invertase

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
5 bound ligands:
Ligand SO4 1 x SO4
Ligand PGE 2 x PGE
Ligand PEG 1 x PEG
Ligand PE8 1 x PE8
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P6122
Unit cell:
a: 98.125Å b: 98.125Å c: 215.534Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.129 0.15
Expression system: Escherichia coli

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