› 7kk8

X-ray diffraction
2.7Å resolution

Fluoride channel Fluc-Ec2 mutant S81T with bromide

Released:
DOI: 10.2210/pdb7kk8/pdb
PDB entry 7kk8 coloured by chain, front view.
PDB entry 7kk8 coloured by chain, side view.
PDB entry 7kk8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.
McIlwain BC, Gundepudi R, Koff BB, Stockbridge RB
OpenAccess logo Elife 10 (2021)
PMID: 34250906

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168365 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoride-specific ion channel FluC Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6J5N4 (Residues: 1-126; Coverage: 100%)
Gene names: AKG29_01220, B6R31_005152, BANRA_05655, C2M16_27610, C4M78_26960, C9160_28010, CF22_004922, CWS33_26140, D9J61_22795, DNQ45_14105, ECS286_0026, EIA08_25210, EIZ93_19245, EN85_004657, EPS76_20210, ExPECSC038_03841, F9413_22620, FEH53_22975, FZU14_25965, G3W53_24200, GAI89_25135, HIA71_25900, HID72_005003, HIE29_005335, HL601_24225, HLZ50_24015, HND12_27530, IFC14_005208, IG021_005385, KTF03_005097, NCTC9001_00147, NCTC9077_06349, OFN31_23365, RCS79_P0115, SAMEA4370473_00090, TUM18780_48590, crcB, crcB_2, flc_2, fluC, pCTXM15_EC8_00123, pO103_22
Sequence domains: CrcB-like protein, Camphor Resistance (CrcB)
monobody M9 Chains: C, D
Molecule details ›
Chains: C, D
Length: 97 amino acids
Theoretical weight: 10.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

5 bound ligands:
Ligand BR 2 x BR
Ligand NA 1 x NA
Ligand DMU 3 x DMU
Ligand F 1 x F
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41
Unit cell:
a: 87.06Å b: 87.06Å c: 143.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.256
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Ion permeation, selectivity, and electronic polarization in fluoride channels.
Yue et al. (2022)
3 more

1 mention without citation

The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.
McIlwain et al. (2021)