› 7kkb

X-ray diffraction
2.9Å resolution

Fluoride channel Fluc-Ec2 mutant S81C with bromide

Released:
DOI: 10.2210/pdb7kkb/pdb
PDB entry 7kkb coloured by chain, front view.
PDB entry 7kkb coloured by chain, side view.
PDB entry 7kkb coloured by chain, top view.
Source organisms:
Primary publication:
The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.
McIlwain BC, Gundepudi R, Koff BB, Stockbridge RB
Elife 10 (2021)
PMID: 34250906

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168368 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoride-specific ion channel FluC Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6J5N4 (Residues: 1-126; Coverage: 100%)
Gene names: AKG29_01220, B6R31_005152, BANRA_05655, BK292_28205, C2M16_27610, CF22_004922, D4U49_23430, D9J61_22795, DNQ45_14105, ECS286_0026, EIA08_25210, EIZ93_19245, EN85_004657, EPS76_20210, EcSP15_n5000, ExPECSC038_03841, F9413_22620, FZU14_25965, G3W53_24200, GAI89_25135, HIE29_005335, HL601_24225, HLZ50_24015, NCTC9001_00147, NCTC9077_06349, RCS79_P0115, SAMEA4370473_00090, crcB, crcB_2, flc_2, fluC, pCTXM15_EC8_00123, pO103_22
Sequence domains: CrcB-like protein, Camphor Resistance (CrcB)
monobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 97 amino acids
Theoretical weight: 10.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand BR 2 x BR
Ligand NA 1 x NA
Ligand F 2 x F
Ligand DMU 3 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41
Unit cell:
a: 87.17Å b: 87.17Å c: 142.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.259
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Ion permeation, selectivity, and electronic polarization in fluoride channels.
Yue et al. (2022)
3 more

1 mention without citation

The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.
McIlwain et al. (2021)