› 7kpp

X-ray diffraction
1.45Å resolution

Structure of the E102A mutant of a GNAT superfamily PA3944 acetyltransferase

Released:
DOI: 10.2210/pdb7kpp/pdb
PDB entry 7kpp coloured by chain, front view.
PDB entry 7kpp coloured by chain, side view.
PDB entry 7kpp coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Gcn5-Related N-Acetyltransferases (GNATs) With a Catalytic Serine Residue Can Play Ping-Pong Too.
Baumgartner JT, Habeeb Mohammad TS, Czub MP, Majorek KA, Arolli X, Variot C, Anonick M, Minor W, Ballicora MA, Becker DP, Kuhn ML
OpenAccess logo Front Mol Biosci 8 646046 (2021)
PMID: 33912589

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyltransferase PA3944 Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22.04 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HX72 (Residues: 1-192; Coverage: 100%)
Gene name: PA3944
Sequence domains: Acetyltransferase (GNAT) domain

Ligands and Environments

3 bound ligands:
Ligand COA 2 x COA
Ligand EDO 7 x EDO
Ligand UNX 8 x UNX
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 36.344Å b: 44.038Å c: 60.184Å
α: 98.03° β: 106.88° γ: 90.03°
R-values:
R R work R free
0.15 0.149 0.168
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective.
Lammers M. (2021)
1 more

2 mentions without citation

Gcn5-Related N-Acetyltransferases (GNATs) With a Catalytic Serine Residue Can Play Ping-Pong Too.
Baumgartner et al. (2021)
1 more