› 7odj

X-ray diffraction
1.3Å resolution

Exo-mannosidase from Cellvibrio mixtus bound to N-alkyl mannocyclophellitol aziridine

Released:
DOI: 10.2210/pdb7odj/pdb
PDB entry 7odj coloured by chain, front view.
PDB entry 7odj coloured by chain, side view.
PDB entry 7odj coloured by chain, top view.
Source organism: Cellvibrio mixtus
Primary publication:
Synthesis of broad-specificity activity-based probes for exo-β-mannosidases.
McGregor NGS, Kuo CL, Beenakker TJM, Wong CS, Offen WA, Armstrong Z, Florea BI, Codée JDC, Overkleeft HS, Aerts JMFG, Davies GJ
OpenAccess logo Org Biomol Chem 20 877-886 (2022)
PMID: 35015006

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Man5A Chain: AAA
Molecule details ›
Chain: AAA
Length: 440 amino acids
Theoretical weight: 50.23 KDa
Source organism: Cellvibrio mixtus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6QT42 (Residues: 26-456; Coverage: 95%)
Gene name: man5A

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand ACT 1 x ACT
Ligand VEE 1 x VEE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 91.815Å b: 102.23Å c: 50.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.125 0.158
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Site-Selective Modification of (Oligo)Saccharides.
Witte et al. (2022)
1 other article