› 7ofx

X-ray diffraction
2.15Å resolution

Crystal structure of a GH31 family sulfoquinovosidase mutant D455N from Agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (SQGro)

Released:
DOI: 10.2210/pdb7ofx/pdb
PDB entry 7ofx coloured by chain, front view.
PDB entry 7ofx coloured by chain, side view.
PDB entry 7ofx coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria.
Sharma M, Lingford JP, Petricevic M, Snow AJD, Zhang Y, Järvå MA, Mui JW, Scott NE, Saunders EC, Mao R, Epa R, da Silva BM, Pires DEV, Ascher DB, McConville MJ, Davies GJ, Williams SJ, Goddard-Borger ED
OpenAccess logo Proc Natl Acad Sci U S A 119 (2022)
PMID: 35074914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfoquinovosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 672 amino acids
Theoretical weight: 75.23 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9CEZ0 (Residues: 1-664; Coverage: 100%)
Gene names: Atu3285, smoI
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand VCW 4 x VCW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 97.534Å b: 168.396Å c: 100.687Å
α: 90° β: 116.53° γ: 90°
R-values:
R R work R free
0.196 0.194 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Implications of hydrogen sulfide in colorectal cancer: Mechanistic insights and diagnostic and therapeutic strategies.
Lin et al. (2023)
1 more

1 mention without citation

Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria.
Sharma et al. (2022)