› 7oh4

Electron Microscopy
3Å resolution

Cryo-EM structure of Drs2p-Cdc50p in the E1 state with PI4P and Mg2+ bound

Released:
DOI: 10.2210/pdb7oh4/pdb
PDB entry 7oh4 coloured by chain, front view.
PDB entry 7oh4 coloured by chain, side view.
PDB entry 7oh4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Basis of Substrate-Independent Phosphorylation in a P4-ATPase Lipid Flippase.
Timcenko M, Dieudonné T, Montigny C, Boesen T, Lyons JA, Lenoir G, Nissen P
J Mol Biol 433 167062 (2021)
PMID: 34023399
Related structures: EMD-12893

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150549 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Phospholipid-transporting ATPase DRS2 Chain: A
Molecule details ›
Chain: A
Length: 1465 amino acids
Theoretical weight: 164.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39524 (Residues: 1-1246, 1247-1355; Coverage: 100%)
Gene names: DRS2, FUN38, SWA3, YAL026C
Sequence domains:
Phospholipid-transporting ATPase accessory subunit CDC50 Chain: C
Molecule details ›
Chain: C
Length: 413 amino acids
Theoretical weight: 47.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25656 (Residues: 1-391; Coverage: 100%)
Gene names: CDC50, SWA4, YCR094W, YCR94W
Sequence domains: LEM3 (ligand-effect modulator 3) family / CDC50 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Ligand NAG 2 x NAG
Ligand BMA 2 x BMA
3 bound ligands:
Ligand 2Y5 1 x 2Y5
Ligand MG 1 x MG
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-12893
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Mechanisms of Non-Vesicular Exchange of Lipids at Membrane Contact Sites: Of Shuttles, Tunnels and, Funnels.
Egea PF. (2021)
4 more