› 7owi

X-ray diffraction
1.7Å resolution

Crystal structure of dimeric chlorite dismutase variant R127A (CCld R127A) from Cyanothece sp. PCC7425

Released:
DOI: 10.2210/pdb7owi/pdb
PDB entry 7owi coloured by chain, front view.
PDB entry 7owi coloured by chain, side view.
PDB entry 7owi coloured by chain, top view.
Source organism: Cyanothece sp. PCC 7425
Primary publication:
Impact of the dynamics of the catalytic arginine on nitrite and chlorite binding by dimeric chlorite dismutase.
Serra I, Schmidt D, Pfanzagl V, Mlynek G, Hofbauer S, Djinović-Carugo K, Furtmüller PG, García-Rubio I, Van Doorslaer S, Obinger C
J Inorg Biochem 227 111689 (2022)
PMID: 34922158

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chlorite dismutase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 188 amino acids
Theoretical weight: 21.74 KDa
Source organism: Cyanothece sp. PCC 7425
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8HNS6 (Residues: 2-182; Coverage: 100%)
Gene name: Cyan7425_1434
Sequence domains: Chlorite dismutase

Ligands and Environments

4 bound ligands:
Ligand HEM 4 x HEM
Ligand PEG 4 x PEG
Ligand MPD 4 x MPD
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 54.447Å b: 72.675Å c: 112.12Å
α: 90° β: 94.835° γ: 90°
R-values:
R R work R free
0.174 0.173 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Roles of High-valent Hemes and pH Dependence in Halite Decomposition Catalyzed by Chlorite Dismutase from Dechloromonas aromatica.
Geeraerts et al. (2022)
1 more