› 7ppe

X-ray diffraction
1.86Å resolution

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 492 amino acids
Theoretical weight: 55.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43490 (Residues: 1-491; Coverage: 100%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 6 x PO4
Ligand 7YK 2 x 7YK
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 60.823Å b: 106.771Å c: 82.625Å
α: 90° β: 96.35° γ: 90°
R-values:
R R work R free
0.15 0.148 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Mechanism research and treatment progress of NAD pathway related molecules in tumor immune microenvironment.
Xu et al. (2022)
2 other articles

1 mention without citation

A Novel NAMPT Inhibitor-Based Antibody-Drug Conjugate Payload Class for Cancer Therapy.
Böhnke et al. (2022)