› 7qgw

X-ray diffraction
1.3Å resolution

Function and Biology Details

Reaction catalysed: 
The recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cathepsin L2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 24.05 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: O60911 (Residues: 114-334; Coverage: 70%)
Gene names: CATL2, CTSL2, CTSU, CTSV, UNQ268/PRO305
Sequence domains: Papain family cysteine protease

Ligands and Environments

4 bound ligands:
Ligand CL 6 x CL
Ligand MPD 5 x MPD
Ligand GOL 6 x GOL
Ligand RN2 2 x RN2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: P43212
Unit cell:
a: 94.24Å b: 94.24Å c: 126.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.196
Expression system: Komagataella phaffii GS115

Quick links

Citations

1 mention without citation

Calpeptin is a potent cathepsin inhibitor and drug candidate for SARS-CoV-2 infections.
Reinke et al. (2023)