› 7qo2

X-ray diffraction
1.77Å resolution

Peptide GAKSAA in complex with human cathepsin V C25A mutant

Released:
DOI: 10.2210/pdb7qo2/pdb
PDB entry 7qo2 coloured by chain, front view.
PDB entry 7qo2 coloured by chain, side view.
PDB entry 7qo2 coloured by chain, top view.
Source organisms:
Entry authors: Loboda J, Sosnowski P, Tusar L, Vidmar R, Vizovisek M, Horvat J, Kosec G, Impens F, Demol H, Turk B, Gevaert K, Turk D

Function and Biology Details

Reaction catalysed: 
The recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130204 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cathepsin L2 Chains: AA, BA
Molecule details ›
Chains: AA, BA
Length: 222 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: O60911 (Residues: 113-334; Coverage: 70%)
Gene names: CATL2, CTSL2, CTSU, CTSV, UNQ268/PRO305
Sequence domains: Papain family cysteine protease
GAKSAA Peptide Chains: PAA, PAB
Molecule details ›
Chains: PAA, PAB
Length: 6 amino acids
Theoretical weight: 505 Da
Source organism: synthetic construct
Expression system: Not provided
EYS Peptide Chain: PB
Molecule details ›
Chain: PB
Length: 3 amino acids
Theoretical weight: 397 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MPD 5 x MPD
Ligand CL 3 x CL
Ligand GOL 3 x GOL
Ligand TFA 1 x TFA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 94.28Å b: 94.28Å c: 126.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.202
Expression systems:
  • Komagataella phaffii GS115
  • Not provided

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