› 7s0g

Electron Microscopy
3.86Å resolution

Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric Gi/s chimera protein

Released:
DOI: 10.2210/pdb7s0g/pdb
PDB entry 7s0g coloured by chain, front view.
PDB entry 7s0g coloured by chain, side view.
PDB entry 7s0g coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis and mechanism of activation of two different families of G proteins by the same GPCR.
Alegre KO, Paknejad N, Su M, Lou JS, Huang J, Jordan KD, Eng ET, Meyerson JR, Hite RK, Huang XY
Nat Struct Mol Biol 28 936-944 (2021)
PMID: 34759376
Related structures: EMD-24790

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138228 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-1 adrenergic receptor Chain: R
Molecule details ›
Chain: R
Length: 508 amino acids
Theoretical weight: 57.96 KDa
Source organism: Meleagris gallopavo
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07700 (Residues: 31-367; Coverage: 63%)
Gene name: ADRB1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 43.37 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P04896 (Residues: 1-3, 384-394; Coverage: 4%)
  • Canonical: P10824 (Residues: 4-343; Coverage: 96%)
Gene names: GNAS, GNAS1, Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

1 bound ligand:
Ligand 5FW 1 x 5FW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.86Å
Relevant EMDB volumes: EMD-24790
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

2 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
1 more

1 mention without citation

Structural basis and mechanism of activation of two different families of G proteins by the same GPCR.
Alegre et al. (2021)