› 7svq

X-ray diffraction
1.75Å resolution

Crystal Structure of L-galactose dehydrogenase from Spinacia oleracea in complex with NAD+

Released:
DOI: 10.2210/pdb7svq/pdb
PDB entry 7svq coloured by chain, front view.
PDB entry 7svq coloured by chain, side view.
PDB entry 7svq coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Structural Characterization of L-Galactose Dehydrogenase: An Essential Enzyme for Vitamin C Biosynthesis.
Vargas JA, Leonardo DA, D'Muniz Pereira H, Lopes AR, Rodriguez HN, Cobos M, Marapara JL, Castro JC, Garratt RC
OpenAccess logo Plant Cell Physiol (2022)
PMID: 35765894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent oxidoreductase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 37.6 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6BDJ2 (Residues: 1-322; Coverage: 100%)
Gene name: GDH
Sequence domains: Aldo/keto reductase family

Ligands and Environments

1 bound ligand:
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 50.02Å b: 54.98Å c: 63.241Å
α: 86.82° β: 69.12° γ: 84.14°
R-values:
R R work R free
0.184 0.182 0.215
Expression system: Escherichia coli

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Citations

2 review citations

Advances in Novel Animal Vitamin C Biosynthesis Pathways and the Role of Prokaryote-Based Inferences to Understand Their Origin.
Duque et al. (2022)
1 more