› 7t0f

X-ray diffraction
2Å resolution

Crystal structure of S25-39 Fab in complex with 4-MeO-KdoOAll

Released:
DOI: 10.2210/pdb7t0f/pdb
PDB entry 7t0f coloured by chain, front view.
PDB entry 7t0f coloured by chain, side view.
PDB entry 7t0f coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Antigen binding by conformational selection in near-germline antibodies.
Blackler RJ, Müller-Loennies S, Pokorny-Lehrer B, Legg MSG, Brade L, Brade H, Kosma P, Evans SV
J Biol Chem 298 101901 (2022)
PMID: 35395245

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214456 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S25-39 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 24.05 KDa
Source organism: Mus musculus
Expression system: Mus musculus
S25-39 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.24 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

1 bound ligand:
Ligand K71 1 x K71
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P21
Unit cell:
a: 52.111Å b: 63.624Å c: 61.636Å
α: 90° β: 97.4° γ: 90°
R-values:
R R work R free
0.217 0.214 0.261
Expression system: Mus musculus

Quick links

Citations

1 review citation

Recent Progress in Antibody Epitope Prediction.
Zeng et al. (2023)
2 other articles