› 7uuo

X-ray diffraction
2.65Å resolution

Crystal structure of aminoglycoside resistance enzyme ApmA H135A mutant, complex with tobramycin and coenzyme A

Released:
DOI: 10.2210/pdb7uuo/pdb
PDB entry 7uuo coloured by chain, front view.
PDB entry 7uuo coloured by chain, side view.
PDB entry 7uuo coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Mechanistic plasticity in ApmA enables aminoglycoside promiscuity for resistance.
Bordeleau E, Stogios PJ, Evdokimova E, Koteva K, Savchenko A, Wright GD
Nat Chem Biol (2023)
PMID: 37973888

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminocyclitol acetyltransferase ApmA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 276 amino acids
Theoretical weight: 31.26 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1D0AST6 (Residues: 1-274; Coverage: 100%)
Gene name: apmA

Ligands and Environments

3 bound ligands:
Ligand TOY 3 x TOY
Ligand EDO 1 x EDO
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3
Unit cell:
a: 108.292Å b: 108.292Å c: 87.726Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.288 0.286 0.328
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Aliskiren restores renal AQP2 expression during unilateral ureteral obstruction by inhibiting the inflammasome.
Wang et al. (2015)