› 7v1x

X-ray diffraction
1.76Å resolution

Difructose dianhydride I synthase/hydrolase (alphaFFase1) from Bifidobacterium dentium in complex with beta-D-fructofuranose

Released:
DOI: 10.2210/pdb7v1x/pdb
PDB entry 7v1x coloured by chain, front view.
PDB entry 7v1x coloured by chain, side view.
PDB entry 7v1x coloured by chain, top view.
Source organism: Bifidobacterium dentium
Primary publication:
Identification of difructose dianhydride I synthase/hydrolase from an oral bacterium establishes a novel glycoside hydrolase family.
Kashima T, Okumura K, Ishiwata A, Kaieda M, Terada T, Arakawa T, Yamada C, Shimizu K, Tanaka K, Kitaoka M, Ito Y, Fujita K, Fushinobu S
OpenAccess logo J Biol Chem 297 101324 (2021)
PMID: 34688653

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF2961 domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 460 amino acids
Theoretical weight: 53.05 KDa
Source organism: Bifidobacterium dentium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L9SN29 (Residues: 1-452; Coverage: 100%)
Gene name: BDLFYP24_02130
Sequence domains: Protein of unknown function (DUF2961)

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand FRU 6 x FRU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 98.439Å b: 156.304Å c: 100.744Å
α: 90° β: 110.09° γ: 90°
R-values:
R R work R free
0.153 0.151 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Computer Simulation to Rationalize "Rational" Engineering of Glycoside Hydrolases and Glycosyltransferases.
Coines et al. (2022)
5 other articles

1 mention without citation

Identification of difructose dianhydride I synthase/hydrolase from an oral bacterium establishes a novel glycoside hydrolase family.
Kashima et al. (2021)