› 7v9b

X-ray diffraction
1.85Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128985 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein epsilon Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62258 (Residues: 1-232; Coverage: 91%)
Gene name: YWHAE
Sequence domains: 14-3-3 protein
Forkhead box protein O3 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43524 (Residues: 248-258; Coverage: 2%)
Gene names: FKHRL1, FOXO3, FOXO3A

Ligands and Environments

4 bound ligands:
Ligand GOL 2 x GOL
Ligand MPD 1 x MPD
Ligand IMD 1 x IMD
Ligand 323 1 x 323
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE PX-BL21
Spacegroup: C2221
Unit cell:
a: 77.88Å b: 81.04Å c: 83.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.189
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

FOXO family isoforms.
Santos et al. (2023)
1 more

2 mentions without citation

Structure of a 14-3-3ε:FOXO3apS253 Phosphopeptide Complex Reveals 14-3-3 Isoform-Specific Binding of Forkhead Box Class O Transcription Factor (FOXO) Phosphoproteins.
Mathivanan et al. (2022)
1 more