› 7x1l

X-ray diffraction
2.28Å resolution

Malate dehydrogenase from Geobacillus stearothermophilus (gs-MDH) delta E311 mutant complexed with Nicotinamide Adenine Dinucleotide (NAD+)

Released:
DOI: 10.2210/pdb7x1l/pdb
PDB entry 7x1l coloured by chain, front view.
PDB entry 7x1l coloured by chain, side view.
PDB entry 7x1l coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Reducing substrate inhibition of malate dehydrogenase from Geobacillus stearothermophilus by C-terminal truncation.
Shimozawa Y, Matsuhisa H, Nakamura T, Himiyama T, Nishiya Y
Protein Eng Des Sel (2022)
PMID: 36208218

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 331 amino acids
Theoretical weight: 35.8 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A143T1U9 (Residues: 1-311; Coverage: 100%)
Gene name: mdh
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 6 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 242.85Å b: 82.724Å c: 136.703Å
α: 90° β: 98.588° γ: 90°
R-values:
R R work R free
0.235 0.233 0.28
Expression system: Escherichia coli

Quick links