› 7xdf

Electron Microscopy
2.72Å resolution

Cryo-EM structures of human mitochondrial NAD(P)+-dependent malic enzyme in a ternary complex with NAD+ and allosteric inhibitor EA

Released:
DOI: 10.2210/pdb7xdf/pdb
PDB entry 7xdf coloured by chain, front view.
PDB entry 7xdf coloured by chain, side view.
PDB entry 7xdf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Suppression of the human malic enzyme 2 modifies energy metabolism and inhibits cellular respiration
Hsieh JY, Chen KC, Wang CH, Liu GY, Ye JA, Chou YT, Lin YC, Lyu CJ, Chang RY, Liu YL, Li YH, Lee MR, Ho MC, Hung HC
Commun Biol 6 548- (2023)
Related structures: EMD-33146

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent malic enzyme, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 584 amino acids
Theoretical weight: 65.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23368 (Residues: 1-584; Coverage: 100%)
Gene name: ME2
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
1 bound ligand:
Ligand JBR 4 x JBR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.72Å
Relevant EMDB volumes: EMD-33146
Expression system: Escherichia coli BL21(DE3)

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