› 7ym1

X-ray diffraction
2.36Å resolution

Structure of SsbA protein in complex with the anticancer drug 5-fluorouracil

Released:
DOI: 10.2210/pdb7ym1/pdb
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PDB entry 7ym1 coloured by chain, front view.
PDB entry 7ym1 coloured by chain, side view.
PDB entry 7ym1 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus ED98
Primary publication:
Crystal Structure of DNA Replication Protein SsbA Complexed with the Anticancer Drug 5-Fluorouracil.
Su HH, Huang YH, Lien Y, Yang PC, Huang CY
OpenAccess logo Int J Mol Sci 24 (2023)
PMID: 37834349

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.65 KDa
Source organism: Staphylococcus aureus subsp. aureus ED98
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0D1JHQ1 (Residues: 1-105; Coverage: 63%)
Gene names: ACR79_09135, AS572_06090, BN1321_80086, CNH36_02070, CV021_06850, DQU50_09180, E1948_01880, E1948_06340, EIH03_03865, EP54_08250, EQ90_08535, ERS391062_01905, G0Z31_04995, G6Y24_06705, GAY54_13430, GF599_07705, GO793_13375, GO814_10580, GO941_16005, GO942_09280, GQX52_00695, GZ164_04755, H2649_05095, LB359_00270, M1K003_0388, NCTC10702_00704, NCTC5664_00336, NCTC7878_00400, NCTC7972_01579, NCTC8317_00319, QU38_04380, SAMEA1466929_01775, SAMEA1531725_01777, SAMEA4552975_01405, SAMEA70153168_00645, SAMEA70245418_01284, ssb, ssbA, ssb_1, ssb_2
Sequence domains: Single-strand binding protein family

Ligands and Environments

2 bound ligands:
Ligand URF 1 x URF
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P41212
Unit cell:
a: 88.091Å b: 88.091Å c: 57.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.258
Expression system: Escherichia coli BL21

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