› 7ysa

X-ray diffraction
1.95Å resolution

Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with UDP-glucose in chain A and UDP-galactose in chain B from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb7ysa/pdb
PDB entry 7ysa coloured by chain, front view.
PDB entry 7ysa coloured by chain, side view.
PDB entry 7ysa coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Yadav S, Bhatia I, Biswal BK

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 34.45 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WN67 (Residues: 1-314; Coverage: 100%)
Gene names: Rv3634c, galE1
Sequence domains: NAD dependent epimerase/dehydratase family

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
4 bound ligands:
Ligand UPG 1 x UPG
Ligand EDO 3 x EDO
Ligand ACT 1 x ACT
Ligand GDU 1 x GDU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 51.222Å b: 76.452Å c: 79.961Å
α: 90° β: 91.97° γ: 90°
R-values:
R R work R free
0.169 0.167 0.209
Expression system: Mycolicibacterium smegmatis

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