› 7zd9

X-ray diffraction
1.89Å resolution

Crystal structure of the E352T mutant of S-adenosyl-L-homocysteine hydrolase from Synechocystis sp. PCC 6803 cocrystallized with adenosine in the presence of Rb+ cations

Released:
DOI: 10.2210/pdb7zd9/pdb
PDB entry 7zd9 coloured by chain, front view.
PDB entry 7zd9 coloured by chain, side view.
PDB entry 7zd9 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Biochemical and structural insights into an unusual, alkali-metal-independent S-adenosyl-L-homocysteine hydrolase from Synechocystis sp. PCC 6803.
Malecki PH, Imiolczyk B, Barciszewski J, Czyrko-Horczak J, Sliwiak J, Gawel M, Wozniak K, Jaskolski M, Brzezinski K
Acta Crystallogr D Struct Biol 78 865-882 (2022)
PMID: 35775986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, C
Molecule details ›
Chains: A, C
Length: 425 amino acids
Theoretical weight: 46.24 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P74008 (Residues: 1-425; Coverage: 100%)
Gene names: ahcY, sll1234
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand NAD 2 x NAD
Ligand ADN 2 x ADN
Ligand CL 2 x CL
Ligand RB 3 x RB
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2221
Unit cell:
a: 121.117Å b: 196.155Å c: 81.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.203
Expression system: Escherichia coli BL21

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