› 7zg5

X-ray diffraction
2Å resolution

The crystal structure of Salmonella TacAT3-DNA complex

Released:
DOI: 10.2210/pdb7zg5/pdb
PDB entry 7zg5 coloured by chain, front view.
PDB entry 7zg5 coloured by chain, side view.
PDB entry 7zg5 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Molecular stripping underpins derepression of a toxin-antitoxin system.
Grabe GJ, Giorgio RT, Wieczór M, Gollan B, Sargen M, Orozco M, Hare SA, Helaine S
Nat Struct Mol Biol (2024)
PMID: 38538913

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281972 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
tRNA-acetylating toxin 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 19.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0F6B5X4 (Residues: 2-175; Coverage: 99%)
Gene names: STM14_3506, a6, tacT3
Sequence domains: Acetyltransferase (GNAT) family
Antitoxin TacA3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 94 amino acids
Theoretical weight: 10.34 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0F6B5X3 (Residues: 5-96; Coverage: 96%)
Gene names: STM14_3505, a6, tacA3
Sequence domains: Protein of unknown function (DUF1778)
tacAT3 DNA operator Chain: E
Molecule details ›
Chain: E
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
tacAT3 DNA operator Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.7 KDa

Ligands and Environments

4 bound ligands:
Ligand COA 2 x COA
Ligand MPD 3 x MPD
Ligand BA 2 x BA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 79.617Å b: 120.096Å c: 172.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.258
Expression system: Escherichia coli BL21(DE3)

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