› 8aju

X-ray diffraction
1.64Å resolution

Crystal structure of the Q65A mutant of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with adenosine in the presence of K+ cations

Released:
DOI: 10.2210/pdb8aju/pdb
PDB entry 8aju coloured by chain, front view.
PDB entry 8aju coloured by chain, side view.
PDB entry 8aju coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Drozdzal P, Wozniak K, Malecki P, Gawel M, Komorowska M, Brzezinski K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: AAA, CCC
Molecule details ›
Chains: AAA, CCC
Length: 472 amino acids
Theoretical weight: 51.68 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I685 (Residues: 1-469; Coverage: 100%)
Gene names: PA0432, ahcY, sahH
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand PO4 2 x PO4
Ligand NAD 2 x NAD
Ligand ADN 2 x ADN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 142.132Å b: 85.925Å c: 125.568Å
α: 90° β: 131.184° γ: 90°
R-values:
R R work R free
0.167 0.166 0.198
Expression system: Escherichia coli

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