› 8ajw

X-ray diffraction
1.82Å resolution

Crystal structure of the Q65N mutant of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with adenosine in the presence of K+ cations

Released:
DOI: 10.2210/pdb8ajw/pdb
PDB entry 8ajw coloured by chain, front view.
PDB entry 8ajw coloured by chain, side view.
PDB entry 8ajw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Drozdzal P, Wozniak K, Malecki P, Gawel M, Komorowska M, Brzezinski K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: AAA, BBB, CCC, DDD
Molecule details ›
Chains: AAA, BBB, CCC, DDD
Length: 472 amino acids
Theoretical weight: 51.72 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I685 (Residues: 1-469; Coverage: 100%)
Gene names: PA0432, ahcY, sahH
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NAD 4 x NAD
Ligand ADN 4 x ADN
Ligand PEG 1 x PEG
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 74.503Å b: 133.618Å c: 100.865Å
α: 90° β: 103.18° γ: 90°
R-values:
R R work R free
0.16 0.16 0.194
Expression system: Escherichia coli

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