› 8b5d

X-ray diffraction
2Å resolution

Exploring the ligand binding and conformational dynamics of receptor domain 1 of the ABC transporter GlnPQ

Released:
DOI: 10.2210/pdb8b5d/pdb
PDB entry 8b5d coloured by chain, front view.
PDB entry 8b5d coloured by chain, side view.
PDB entry 8b5d coloured by chain, top view.
Source organism: Lactococcus lactis
Entry authors: Whittaker J, Guskov A

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transmembrane type-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.51 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2X0R690 (Residues: 27-251; Coverage: 33%)
Gene names: AMHIJAGA_02474, glnP
Sequence domains: Bacterial extracellular solute-binding proteins, family 3

Ligands and Environments

5 bound ligands:
Ligand MPD 1 x MPD
Ligand GOL 4 x GOL
Ligand GLN 2 x GLN
Ligand NA 4 x NA
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 42.61Å b: 91.5Å c: 57.91Å
α: 90° β: 107.81° γ: 90°
R-values:
R R work R free
0.2 0.196 0.263
Expression system: Escherichia coli

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