› 8bes

X-ray diffraction
1.86Å resolution

Structure of D188A-fructofuranosidase from Rhodotorula dairenensis in complex with fructose

Released:
DOI: 10.2210/pdb8bes/pdb
PDB entry 8bes coloured by chain, front view.
PDB entry 8bes coloured by chain, side view.
PDB entry 8bes coloured by chain, top view.
Source organism: Rhodotorula dairenensis
Primary publication:
Insights into the Structure of the Highly Glycosylated Ffase from Rhodotorula dairenensis Enhance Its Biotechnological Potential.
Jiménez-Ortega E, Narmontaite E, González-Pérez B, Plou FJ, Fernández-Lobato M, Sanz-Aparicio J
OpenAccess logo Int J Mol Sci 23 (2022)
PMID: 36499311

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Beta-fructofuranosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 675 amino acids
Theoretical weight: 70.67 KDa
Source organism: Rhodotorula dairenensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A856TAI5 (Residues: 1-675; Coverage: 100%)
Gene name: INV
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand MAN 13 x MAN
Ligand NAG 28 x NAG
Ligand BTB 4 x BTB
Ligand FRU 4 x FRU
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 80.846Å b: 112.693Å c: 139.617Å
α: 90° β: 104.9° γ: 90°
R-values:
R R work R free
0.186 0.184 0.217
Expression system: Komagataella pastoris

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