› 8c48

X-ray diffraction
1.4Å resolution

Crystal structure of Thermothelomyces thermophila GH30 (double mutant EE) in complex with xylopentaose

Released:
DOI: 10.2210/pdb8c48/pdb
PDB entry 8c48 coloured by chain, front view.
PDB entry 8c48 coloured by chain, side view.
PDB entry 8c48 coloured by chain, top view.
Source organism: Thermothelomyces
Primary publication:
Structural and molecular insights into a bifunctional glycoside hydrolase 30 xylanase specific to glucuronoxylan.
Pentari C, Kosinas C, Nikolaivits E, Dimarogona M, Topakas E
Biotechnol Bioeng (2024)
PMID: 38678481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
GH30 family xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 51.73 KDa
Source organism: Thermothelomyces
Expression system: Komagataella pastoris
UniProt:
  • Canonical: G2Q1N4 (Residues: 20-477; Coverage: 100%)
Gene names: MYCTH_38558, Xyn30A
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand EDO 2 x EDO
Ligand F 1 x F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 40.95Å b: 107.806Å c: 88.241Å
α: 90° β: 95.47° γ: 90°
R-values:
R R work R free
0.129 0.128 0.156
Expression system: Komagataella pastoris

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