› 8cfx

X-ray diffraction
1.98Å resolution

Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with F2X-Entry library fragment H06

Released:
DOI: 10.2210/pdb8cfx/pdb
PDB entry 8cfx coloured by chain, front view.
PDB entry 8cfx coloured by chain, side view.
PDB entry 8cfx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Malecki PH, Gawel M, Stepniewska M, Brzezinski K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D, G, H, I, J
Molecule details ›
Chains: A, B, C, D, G, H, I, J
Length: 472 amino acids
Theoretical weight: 51.74 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I685 (Residues: 1-469; Coverage: 100%)
Gene names: PA0432, ahcY, sahH
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand NAD 8 x NAD
Ligand ADE 8 x ADE
Ligand UID 6 x UID
Ligand K 8 x K
Ligand PO4 26 x PO4
Ligand DMS 6 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P2
Unit cell:
a: 109.574Å b: 103.99Å c: 176.086Å
α: 90° β: 99.58° γ: 90°
R-values:
R R work R free
0.168 0.168 0.203
Expression system: Escherichia coli

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