› 8dk0

X-ray diffraction
1.55Å resolution

Crystal structure of RPA3624, a beta-propeller lactonase from Rhodopseudomonas palustris, with active-site bound (S)gamma-valerolactone

Released:
DOI: 10.2210/pdb8dk0/pdb
PDB entry 8dk0 coloured by chain, front view.
PDB entry 8dk0 coloured by chain, side view.
PDB entry 8dk0 coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Primary publication:
A broad specificity β-propeller enzyme from Rhodopseudomonas palustris that hydrolyzes many lactones including γ-valerolactone.
Hall BW, Bingman CA, Fox BG, Noguera DR, Donohue TJ
OpenAccess logo J Biol Chem 102782 (2022)
PMID: 36502920

Function and Biology Details

Reaction catalysed: 
D-glucono-1,5-lactone + H(2)O = D-gluconate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SMP-30/Gluconolactonase/LRE-like region domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 33.13 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6N3R9 (Residues: 2-309; Coverage: 100%)
Gene names: RPA3624, TX73_018780
Sequence domains: SMP-30/Gluconolactonase/LRE-like region

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand NA 1 x NA
Ligand YVR 1 x YVR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 47.51Å b: 47.51Å c: 199.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.21
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

A broad specificity β-propeller enzyme from Rhodopseudomonas palustris that hydrolyzes many lactones including γ-valerolactone.
Hall et al. (2023)