› 8gnk

Electron Microscopy
3.1Å resolution

CryoEM structure of cytosol-facing, substrate-bound ratGAT1

Released:
DOI: 10.2210/pdb8gnk/pdb
PDB entry 8gnk coloured by chain, front view.
PDB entry 8gnk coloured by chain, side view.
PDB entry 8gnk coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of GABA transporter 1 reveals substrate recognition and transport mechanism.
Nayak SR, Joseph D, Höfner G, Dakua A, Athreya A, Wanner KT, Kanner BI, Penmatsa A
Nat Struct Mol Biol (2023)
PMID: 37400654
Related structures: EMD-34167

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273416 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium- and chloride-dependent GABA transporter 1 Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 63.2 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: P23978 (Residues: 39-599; Coverage: 94%)
Gene names: Gabt1, Gat-1, Gat1, Slc6a1
Sequence domains: Sodium:neurotransmitter symporter family
Fragment antigen binding light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
fragment antigen binding 9D5 heavy chain Chain: H

Ligands and Environments

6 bound ligands:
Ligand NAG 2 x NAG
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand ABU 1 x ABU
Ligand CLR 6 x CLR
Ligand PTY 1 x PTY
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-34167
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

6 citation in other articles

Probing binding and occlusion of substrate in the human creatine transporter-1 by computation and mutagenesis.
Clarke et al. (2024)
5 more