› 8ovn

X-ray diffraction
2.6Å resolution

X-ray structure of the SF-iGluSnFR-S72A

Released:
DOI: 10.2210/pdb8ovn/pdb
PDB entry 8ovn coloured by chain, front view.
PDB entry 8ovn coloured by chain, side view.
PDB entry 8ovn coloured by chain, top view.
Source organisms:
Entry authors: Tarnawski M, Hellweg L, Bergner A, Hiblot J, Leippe P, Johnsson K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate/aspartate import solute-binding protein; Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 518 amino acids
Theoretical weight: 58.02 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P37902 (Residues: 28-294; Coverage: 95%)
  • Canonical: P42212 (Residues: 1-148, 149-150, 151-162; Coverage: 68%)
Gene names: GFP, JW5092, b0655, gltI, ybeJ, yzzK
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 106.14Å b: 106.14Å c: 108.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.253
Expression system: Escherichia coli

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