› 8oy6

X-ray diffraction
2.35Å resolution

Function and Biology Details

Reaction catalysed: 
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235649 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Deoxyribodipyrimidine photo-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 498 amino acids
Theoretical weight: 57.04 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PYK9 (Residues: 1-464; Coverage: 100%)
Gene name: MM_0852
Sequence domains: DNA photolyase
CPD-COMPRISING OLIGONUCLEOTIDE Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
Source organism: synthetic construct
Expression system: Not provided
COUNTERSTRAND-OLIGONUCLEOTIDE Chains: D, F
Molecule details ›
Chains: D, F
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand FDA 2 x FDA
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SwissFEL ARAMIS BEAMLINE ESA
Spacegroup: P212121
Unit cell:
a: 70.2Å b: 117.76Å c: 170.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.287 0.357
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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