› 8pak

X-ray diffraction
1.42Å resolution

Crystal Structure of a Squalene-Hopene Cyclase from Cystobacter fuscus

Released:
DOI: 10.2210/pdb8pak/pdb
PDB entry 8pak coloured by chain, front view.
PDB entry 8pak coloured by chain, side view.
PDB entry 8pak coloured by chain, top view.
Source organism: Cystobacter fuscus DSM 2262
Entry authors: Worthy HL, Mitchell DE, Isupov MN, Littlechild JA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246174 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene cyclase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 39.65 KDa
Source organism: Cystobacter fuscus DSM 2262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A250J6H4 (Residues: 1-361; Coverage: 100%)
Gene name: CYFUS_004654
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ACT 2 x ACT
Ligand EDO 5 x EDO
Ligand MPD 1 x MPD
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 48.244Å b: 75.872Å c: 91.742Å
α: 90° β: 94.269° γ: 90°
R-values:
R R work R free
0.168 0.166 0.205
Expression system: Escherichia coli BL21

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