› 8pun

X-ray diffraction
2.3Å resolution

Old Yellow Enzyme from the thermophilic Ferrovum sp. JA12

Released:
DOI: 10.2210/pdb8pun/pdb
PDB entry 8pun coloured by chain, front view.
PDB entry 8pun coloured by chain, side view.
PDB entry 8pun coloured by chain, top view.
Source organism: Ferrovum sp. JA12
Entry authors: Polidori N, Gruber K

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 373 amino acids
Theoretical weight: 40.76 KDa
Source organism: Ferrovum sp. JA12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R0LNE8 (Residues: 1-354; Coverage: 100%)
Gene names: FERRO_10540, namA
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
7 bound ligands:
Ligand PEG 3 x PEG
Ligand 9D2 2 x 9D2
Ligand YT3 7 x YT3
Ligand CL 5 x CL
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P4122
Unit cell:
a: 187.712Å b: 187.712Å c: 129.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.205
Expression system: Escherichia coli BL21(DE3)

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