› 8qxq

X-ray diffraction
0.94Å resolution

PsiM in complex with SAH and psilocybin

Released:
DOI: 10.2210/pdb8qxq/pdb
PDB entry 8qxq coloured by chain, front view.
PDB entry 8qxq coloured by chain, side view.
PDB entry 8qxq coloured by chain, top view.
Source organism: Psilocybe cubensis
Primary publication:
Methyl transfer in psilocybin biosynthesis.
Hudspeth J, Rogge K, Dörner S, Müll M, Hoffmeister D, Rupp B, Werten S
OpenAccess logo Nat Commun 15 2709 (2024)
PMID: 38548735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Psilocybin synthase Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.89 KDa
Source organism: Psilocybe cubensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DPA9 (Residues: 1-309; Coverage: 100%)
Gene name: psiM
Sequence domains: RNA methyltransferase

Ligands and Environments

3 bound ligands:
Ligand SAH 1 x SAH
Ligand X8Q 1 x X8Q
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 49.342Å b: 78.521Å c: 83.925Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.149
Expression system: Escherichia coli BL21(DE3)

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