› 8smn

Electron Microscopy
3.2Å resolution

Xenopus laevis hyaluronan synthase 1, nascent HA polymer bound state

Released:
DOI: 10.2210/pdb8smn/pdb
PDB entry 8smn coloured by chain, front view.
PDB entry 8smn coloured by chain, side view.
PDB entry 8smn coloured by chain, top view.
Source organisms:
Entry authors: Gorniak I, Zimmer J
Related structures: EMD-40594

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243740 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Hyaluronan synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 601 amino acids
Theoretical weight: 70.39 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P13563 (Residues: 1-588; Coverage: 100%)
Gene name: has1
Sequence domains: Glycosyltransferase like family 2
Fab15 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 234 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Fab15 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BDP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-40594
Expression system: Spodoptera frugiperda

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