› 8t3o

Electron Microscopy
3.06Å resolution

Cryo-EM structure of the TUG-891 bound FFA4-Gq complex

Released:
DOI: 10.2210/pdb8t3o/pdb
PDB entry 8t3o coloured by chain, front view.
PDB entry 8t3o coloured by chain, side view.
PDB entry 8t3o coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the ligand recognition and signaling of free fatty acid receptors.
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C
OpenAccess logo Sci Adv 10 eadj2384 (2024)
PMID: 38198545
Related structures: EMD-41007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-238945 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Free fatty acid receptor 4 Chain: R
Molecule details ›
Chain: R
Length: 297 amino acids
Theoretical weight: 33.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5NUL3 (Residues: 22-328; Coverage: 82%)
Gene names: FFAR4, GPR120, GPR129, O3FAR1, PGR4
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 342 amino acids
Theoretical weight: 37.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 57 amino acids
Theoretical weight: 6.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 6-62; Coverage: 80%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(q) Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
scFv16 Chain: N
Molecule details ›
Chain: N
Length: 266 amino acids
Theoretical weight: 28.63 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

3 bound ligands:
Ligand YN9 1 x YN9
Ligand 2Y5 1 x 2Y5
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-41007
Expression system: Spodoptera frugiperda

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