› 8vw2

X-ray diffraction
1.95Å resolution

Crystal Structure of Apo UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from E. coli (ATP bound)

Released:
DOI: 10.2210/pdb8vw2/pdb
PDB entry 8vw2 coloured by chain, front view.
PDB entry 8vw2 coloured by chain, side view.
PDB entry 8vw2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoylalanine--D-glutamate ligase Chain: A
Molecule details ›
Chain: A
Length: 445 amino acids
Theoretical weight: 47.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14900 (Residues: 1-438; Coverage: 100%)
Gene names: JW0086, b0088, murD
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand CA 3 x CA
Ligand ATP 1 x ATP
Ligand MG 4 x MG
Ligand MPD 1 x MPD
Ligand DMS 1 x DMS
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 58.456Å b: 69.944Å c: 100.534Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.225
Expression system: Escherichia coli BL21(DE3)

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