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PDBsum entry 1vbk
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protein ligands Protein-protein interface(s) links
Structural genomics, unknown function PDB id
1vbk

 

 

 

 

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Contents
Protein chains
307 a.a. *
Ligands
MRD ×4
Waters ×637
* Residue conservation analysis
PDB id:
1vbk
Name: Structural genomics, unknown function
Title: Crystal structure of ph1313 from pyrococcus horikoshii ot3
Structure: Hypothetical protein ph1313. Chain: a, b. Engineered: yes
Source: Pyrococcus horikoshii. Organism_taxid: 70601. Strain: ot3. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
1.90Å     R-factor:   0.199     R-free:   0.222
Authors: M.Sugahara,N.Kunishima,Riken Structural Genomics/proteomics Initiative (Rsgi)
Key ref:
M.Sugahara et al. (2007). Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 56-58. PubMed id: 17183176 DOI: 10.1107/S1744309106054509
Date:
27-Feb-04     Release date:   19-Apr-05    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
O59053  (O59053_PYRHO) -  THUMP domain-containing protein from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Seq:
Struc: 		307 a.a.
307 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1107/S1744309106054509 Acta Crystallogr Sect F Struct Biol Cryst Commun 63:56-58 (2007)
PubMed id: 17183176  
 
 
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3.
M.Sugahara, S.Murai, M.Sugahara, N.Kunishima.
 
  ABSTRACT  
 
The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 A resolution and a single anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1 A resolution were collected using synchrotron radiation at 100 K. The native crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.7, b = 71.2, c = 141.8 A.
 
  Selected figure(s)  
 
Figure 1.
Crystals of PH1313 from P. horikoshii OT3. The crystals have approximate dimensions of 0.15 x 0.10 x 0.01 mm. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 January 1; 63(Pt 1): 56–58. Published online 2006 December 22. doi: 10.1107/S1744309106054509. Copyright [copyright] International Union of Crystallography 2007
 
  The above figure is reprinted from an Open Access publication published by the IUCr: Acta Crystallogr Sect F Struct Biol Cryst Commun (2007, 63, 56-58) copyright 2007.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
  18997348 A.P.Silva, R.T.Byrne, M.Chechik, C.Smits, D.G.Waterman, and A.A.Antson (2008).
Expression, purification, crystallization and preliminary X-ray studies of the TAN1 orthologue from Methanothermobacter thermautotrophicus.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 1083-1086.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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