PDBsum entry 4dpy

Lyase/lyase inhibitor

PDB id: 4dpy

Contents

Protein chains

330 a.a.

Ligands

2P0 ×2

Waters ×399

PDB id:

4dpy

Name:

Lyase/lyase inhibitor

Title:

Crystal structure of staphylococcus epidermidis s192a mevalonate diphosphate decarboxylase complexed with inhibitor dpgp

Structure:

Mevalonate diphosphate decarboxylase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Staphylococcus epidermidis. Organism_taxid: 1282. Gene: mvad. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.14Å R-factor: 0.171 R-free: 0.226

Authors:

M.L.Barta,W.J.Mcwhorter,B.V.Geisbrecht

Key ref:

M.L.Barta et al. (2012). Structural basis for nucleotide binding and reaction catalysis in mevalonate diphosphate decarboxylase. Biochemistry, 51, 5611-5621. PubMed id: 22734632

Date:

14-Feb-12 Release date: 11-Jul-12

Protein chains

Q9FD73  (Q9FD73_STAEP) -  diphosphomevalonate decarboxylase from Staphylococcus epidermidis

Seq: 327 a.a.

Struc: 330 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.1.33 - diphosphomevalonate decarboxylase.
• Pathway: Terpenoid biosynthesis
• Reaction: (R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2

(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate (Bound ligand (Het Group name = 2P0) matches with 47.83% similarity) + CO2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 51:5611-5621 (2012)

PubMed id: 22734632

Structural basis for nucleotide binding and reaction catalysis in mevalonate diphosphate decarboxylase.

M.L.Barta, W.J.McWhorter, H.M.Miziorko, B.V.Geisbrecht.

ABSTRACT

No abstract given.