PDBsum entry 8slg

Ligase

PDB id: 8slg

Contents

Protein chains

429 a.a.

Ligands

G5A ×2

GOL ×3

Metals

_MG ×2

_ZN ×2

Waters ×289

PDB id:

8slg

Name:

Ligase

Title:

Crystal structure of glycine tRNA ligase from mycobacterium thermoresistibile (glycyl adenylate bound)

Structure:

Glycine--tRNA ligase. Chain: a, b. Engineered: yes

Source:

Mycolicibacterium thermoresistibile atcc 19527. Organism_taxid: 1078020. Gene: glyqs. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.95Å R-factor: 0.176 R-free: 0.201

Authors:

Seattle Structural Genomics Center For Infectious Disease,Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

L.Liu et al. Crystal structure of glycine tRNA ligase from mycobac thermoresistibile (glycyl adenylate bound). To be published, . PubMed id: 40587479

Date:

21-Apr-23 Release date: 03-May-23

Protein chains

G7CIG9  (G7CIG9_MYCT3) -  Glycine--tRNA ligase from Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316)

Seq: 461 a.a.

Struc: 429 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.1.1.14 - glycine--tRNA ligase.
• Reaction: tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate

tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP (Bound ligand (Het Group name = G5A) matches with 61.29% similarity) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site